Biblioteca virtual en Vacunas  
Biblioteca virtual en Vacunas
Biblioteca Virtual en Vacunas
Red Latinoamericana de Información Científico Técnico en Vacunas
 
 
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Martes, 17/9/2019

VacciMonitor
Biotecnología Aplicada


Resumen
The current antimeningococcal vaccine, composed of the purified capsular polysaccharide of group C (PS-C) does not induce a good antibody response in children under two years of age. This drawback has usually been overcome by coupling immunogenic proteins to PS or oligosaccharides to convert these antigens to a T-dependent form. In this study the purified PSC was depolymerized by acid hydrolysis to obtain oligosaccharides C (OS-C), and it was evaluated the integrity of their antigenic properties by inhibition ELISA. Twenty OS-C were obtained by this experimental procedure. The molecular size of the meningococcal OS-C was determined by gel filtration. Significant differences between the molecular size of the OS-C were obtained with different pH and temperature values. An inhibition ELISA was developed in order to evaluate the antigenic properties of the OS-C obtained. The antigenic properties were dependent on the molecular size. Oligosaccharides with the same antigenic properties as Neisseria meningitidis PS-C were obtained by acid hydrolysis at 60 °C, pH 4 for 20 min.
 

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